Media development, critical process parameters development for high expression and better product quality, tech transfer and process scale-up.  Serum-free media development in shake flask for screening of various basal and feed media. Evaluation of expression level and product quality.  Lab scale process development in shake flask for optimization of 4-5 process parameters. Three bioreactor runs to establish lab scale cell culture process. Titer of lab scale process: not less than 3g/L.